This page provides a detailed comparison of the specifications for the John Deere 300B and the John Deere 401B.
| John Deere 300B | John Deere 401B | |
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| Engine | ||
| Engine Manufacturer | John Deere | John Deere |
| Engine Cooling Type | Liquid-cooled | Liquid-cooled |
| Engine Fuel Type | Gasoline | Diesel |
| Engine Induction Type | Naturally aspirated | Naturally aspirated |
| Engine Cylinders | 3 | 4 |
| Engine Displacement Value | 152 ci (2.5 L) | 219 ci (3.6 L) |
| Engine Rated Rpm | 2500 | 2500 |
| Engine Power Output Value | 43 hp (32.1 kW) | 66 hp (49.2 kW) |
| Transmission | ||
| Transmission Type | Power shuttle | Power shuttle with hydraulic wet disc clutch |
| Transmission Forward Gears | 8 | 8 |
| Transmission Reverse Gears | 8 | 4 |
| Dimensions | ||
| Length Value | 183.9 | 190.9 |
| Width Value | 79.9 | 74.8 |
| Operating Weight Value | 7449 lb (3379 kg) | 4451 lb (2019 kg) |
| Miscellaneous | ||
| Chassis | 4x2 2WD | 4x2 2WD |
| Drive Type | 2WD | 2WD |
| Front Tire Type | - | - |
| Rear Tire Type | - | - |
| Hitch Category | - | Optional |
| Hydraulic System Type | Closed center | Closed center |
| Operator Station Type | Four-post ROPS | Open operator station or ROPS |
| Starter Type | Electric | Electric |
